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Early embryonic death of calves due to sub-fertility in cows is of great economic concern to dairy industry. Early pregnancy factor (EPF) is a secretory protein with pregnancy associated immunosuppressive properties. Rosette inhibition test (RIT) was used to detect EPF in inseminated dairy cows. Blood samples were collected at two intervals, 1-3 and 5-7 days after insemination from 23 inseminated and 18 non-inseminated control cows for RIT and pregnancy diagnosis performed between 42 and 45 days on palpation. The study indicates that RIT (P<0.05) has the potential to distinguish pregnant from non-pregnant dairy cows in the first week of pregnancy.


V Ghaffari Laleh, R Ghaffari Laleh, N Pirany, M Moghadaszadeh Ahrabi. Measurement of EPF for detection of cow pregnancy using rosette inhibition test. Theriogenology. 2008 Jul 1;70(1):105-7

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PMID: 18433850

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