Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

Garlic has been known for its therapeutic effects for centuries and is used worldwide as a functional food. The concentration of the active molecules could be enhanced by a better knowledge of their biosynthesis. The precursor of these compounds, alliin (a sulfur amino-acid) has been obtained by chemical synthesis. However, this synthesis route also leads to a diastereoisomer as co-product. This work describes the development of an analytical method which allows the separation and quantification of the two diastereoisomers in order to determine in which proportion the natural form can be produced. The HPLC method which was optimized and validated by accuracy profile exploits an original stationary phase consisting of porous graphitic carbon (PGC). Furthermore, the developped method was used to separate the diastereoisomers of methiin, another cysteine sulfoxide, and to analyze an aqueous extract of garlic. The ability to quantify the amount of natural alliin is valuable for further work on garlic molecules and their application for health protection. Copyright © 2012 Elsevier B.V. All rights reserved.

Citation

Bérénice Dethier, Morgan Laloux, Emilien Hanon, Katherine Nott, Stephanie Heuskin, Jean-Paul Wathelet. Analysis of the diastereoisomers of alliin by HPLC. Talanta. 2012 Nov 15;101:447-52

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 23158347

View Full Text